“© Check List : ty ® PENSUFT. Check List 19 (5): 735-741 https://doi.org/10.15560/19.5.735 NOTES ON GEOGRAPHIC DISTRIBUTION First record of Gargaphia paula Drake, 1939 (Hemiptera, Tingidae) from Colombia, with a new host plant and an updated checklist of Gargaphia species in the country JEAN GAMBOA!” , CAROLINA FONTECHA’, ERIKA VALENTINA VERGARA-NAVARRO”?, YENNIFER ANDREA CARRENO-GUEVARA], JOHN QUIROZ’*, FRANCISCO SERNA” 1 Laboratorio de Entomologia Universidad de la Amazonia (LEUA), Universidad de la Amazonia, Florencia, Caqueta, Colombia « JG: j.gamboa@udla.edu.co ® https://orcid.org/0000-0002-8783-0175 « YACG: y.carreno@udla.edu.co ® https://orcid.org/0000- 0001-7779-3633 2 Museo Entomoldégico UNAB, Universidad Nacional de Colombia sede Bogota, Bogota D.C., Colombia « CF: dcfontechaca@unal.edu. co ® https://orcid.org/0009-0005-1866-4344 « EVVN: evvergara@agrosavia.co ® https://orcid.org/0000-0002-8447-6844 « FS: fisernac@unal.edu.co ® https://orcid.org/0000-0002-6143-9821 3 Coleccién Taxondmica Nacional de Insectos “Luis Maria Murillo” (CTNI), Corporacién Colombiana de Investigacion Agropecuaria AGROSAVIA, Mosquera, Cundinamarca, Colombia 4 Museo Entomoldgico “Francisco Luis Gallego” (MEFLG), Universidad Nacional de Colombia sede Medellin, Medellin, Antioquia, Colombia « JQ: jaquirozg@unal.edu.co @ http://orcid.org/0009-0008-1551-0405 * Corresponding author Abstract. Gargaphia paula Drake, 1939 is recorded for the first time for Colombia in two extreme localities, one in the northern region of the country and the other in the southeast. Moreover, it was found on Calopogonium the journal of biodiversity data mucunoides Desv. (Fabaceae), a new host plant recorded for the species. Keywords. Amazonia, distribution, host, lacebug, phytophagous Academic editor: Hélcio Gil-Santana Received 14 July 2023, accepted 19 September 2023, published 20 October 2023 Gamboa J, Fontecha C, Vergara-Navarro EV, Carreho-Guevara YA, Quiroz J, Serna F (2023) First record of Gargaphia paula Drake, 1939 (Hemiptera, Tingidae) from Colombia, with a new host plant and an updated checklist of Gargaphia species in the country. Check List 19 (5): 735-741. https://doi.org/10.15560/19.5.735 Introduction Species of the genus Gargaphia Stal, 1862 (Tingidae, Tinginae, Tingini) are distributed in the Western Hemisphere (Drake and Ruhoff 1960), and while host plants for roughly half of the species are unknown, the families Fabaceae, Solanaceae, Malvaceae, Euphorbi- aceae, Asteraceae, and Rosaceae constitute the most common host plants for Gargaphia spp. (Drake and Ruhoff 1965). Gargaphia paula Drake, 1939 has previously been recorded from Brazil (Acre state), Costa Rica (Pun- tarenas province), Ecuador (Los Rios and Zamora- Chinchipe provinces), Panama (Canal Zone), and Peru (Tingo Maria) (Drake 1939; Drake and Ruhoff 1965; Guidoti et al. 2014). Host plants of G. paula include six species of the genus Arachis L. (Fabaceae) and hybrids of those species (Guidoti et al. 2014). Methods Two specimens, a male and a female of Gargaphia paula, were collected by FS from the underside of leaves of Arachis pintoi Krapov. & W.C. Gregory (Fabaceae) in Florencia (Caqueta, Colombia). In another local- ity in the same city, 18 additional specimens of G. paula were collected by JG and M. Silva, a contributor researcher, on the same host plant. With the help of a fine-tipped paintbrush, the collected individuals were placed into a 30 mL plastic bottle containing 96% ethyl alcohol and taken to the Museo Entomolégico UNAB in Bogota, and Laboratorio de Entomologia Universi- ©The authors. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 736 dad de la Amazonia (LEUA) in Florencia, Caqueta for identification, photographic registry, and curatorship. The curatorship of specimens was carried out following the protocols established in the collections of UNAB (Martinez-Alava and Serna 2015) and LEUA. Two and 18 specimens were deposited in UNAB and LEUA col- lections, respectively. The preserved specimens were studied employing an Olympus SZ61 stereomicroscope at 90x magnifica- tion. Gargaphia paula was identified by comparing the new materials to the original description of the species by Drake (1939) anda photograph of the holotype avail- able online (Smithsonian Institution, National Muse- um of Natural History 2023; https://www.gbif.org/ occurrence/1321645521). Photographs were taken with a Leica M205A stereomicroscope with a built-in camera and a Hitachi TM4000Plus II environmental scanning electron microscope. A distribution map of G. paula in the neotropics was plotted with QGIS v. S262: A complete and detailed search of the species was Check List 19 (5) carried out in Colombian agricultural entomological collections, including Coleccién Taxonémica Nacional de Insectos “Luis Maria Murillo” (CTNI) of Agrosavia, finding 12 more specimens of G. paula collected in 1986 in the northern Caribbean municipality of Cienaga, Magdalena, Colombia, with an identification labeled by Froeschner in 1987. Results Gargaphia paula Drake, 1939 Figures 1-5 Materials examined. COLOMBIA - Caqueta « Floren- cia, Barrio Siete de Agosto, Terminal de transporte ter- restre; 01°37'11"N, 075°36'35"W; 281 m alt.; 16.X1.2022; F. Serna leg.; manual collection, underside of leaves of Arachis pintoi (Fabaceae), mani forrajero; UNAB 6531, 1 ¢ & 1 & « Florencia; Barrio Porvenir, Universidad de la Amazonia Sede Principal; 01°37'09"N, 075°36'15" W; 242 m alt.; 05.V.2023; J. Gamboa & M. Silva leg.; man- ual collection, underside of leaves of Arachis pintoi Figure 1. Gargaphia paula Drake, 1939, male specimen from Colombia (LEUA 58428). A. Dorsal view. B. Ventral view. C. Lateral view. Gamboa et al. | Gargaphia paula from Colombia 737 Figure 2. Gargaphia paula Drake, 1939, female specimen from Colombia (LEUA 58417). A. Dorsal view. B. Ventral view. C. Lateral view. (Fabaceae), mani forrajero; LEUA 58385, 58412-58428, 13 ¢ & 5 9. - Magdalena « Cienaga; X.1986; F. Posa- da leg.; manual collection, on leaves of Calopogonium mucunoides (Fabaceae), calopo; CTNI 3848, 5 d &7 9. Identification. Gargaphia paula resembles Leptopharsa lineata (Champion, 1897), but the former has the ven- tral rostral channel interrupted posteriorly at the meso- sternum level. Moreover, G. paula can be misidentified as G. schulzei Drake, 1954, due to their similar traits (Guidoti et al. 2014). Specimens found in this survey match well the orig- inal description by Drake (1939: 68): “Elytra with an oblique fuscous band near the apex. Head black, with five, pale, testaceous spines, the median and hind pair longer. Antenna slender, moderately long; segments I and II black, the former stouter and three times as long as the latter; III long, testaceous, two and one-half times as long as IV; IV long, the distal three-fourths black. Pronotum black, the triangular portion testaceous. Carinae foliaceous, testaceous, uniseriate; lateral cari- nae not quite extending as far forward as the base of the hood, slightly converging behind or parallel; median carina slightly more elevated. Hood moderately large, roof-shaped above, highest in front, projecting slight- ly forward in front. Paranota moderately broad, testa- ceous, biseriate, the outer margin rounded, and slightly convergent behind. Labium extending to the interrupt- ed channel. Elytra very similar in appearance and mark- ings to L. lineata (Champ.); costal are mostly biseriate, triseriate in widest part, and areola hyaline; subcostal area broad, triseriate in the widest part. Body beneath black” (Figs. 1-3). In most specimens from Colombia, the lateral carina of the discal area possesses two maculae at the summit, a one-celled anterior testaceous and a two-celled black posterior one (Fig. 1-3). 738 Check List 19 (5) TM4000 15 Ped 2 500um TM4000 15kV X100 SE L TM4000 15kV X60 SE L Figure 3. Gargaphia paula Drake, 1939, male specimen from Colombia (LEUA-58428). A. Head. B. Labium. C. Antenna. D. Paranota. E. Elytra. Discussion respectively (see Morrone 2014). These new records indi- cate that G. paula is a widely distributed species adapt- Two new Colombian localities for Gargaphia paula ed to different environments and that it might be found from the towns of Cienaga and Florencia inthe northern- throughout Colombian territory. These new records help most and southernmost regions of the country, respec- fill gap in the distribution of G. paula between Central tively, are belong to two rather different biogeograph- and South America (Fig. 4). Eleven species of the genus ic provinces, Guajira (Cienaga) and Napo (Florencia), | Gargaphia are now recorded from Colombia (Table 1). Gamboa et al. | Gargaphia paula from Colombia 739 74°00'00"W | 68°00'00"W 11°00’00"N ~N00,00011 6°00'00"N 'N,00,0009 1°00'00"N N.00,0001 ” = = S S ie) t S100,000F @ Previous records @ New records 9°00'00"S $100,0006 6°00'00"" | 68°00'00"W Figure 5. Host plants of Gargaphia paula Drake, 1939. A. Arachis pintoi Krapov. & W.C. Greg. (Fabaceae). B. Calopogonium mucunoi- des Desv. (Fabaceae) (new host plant record). 740 Check List 19 (5) Table 1. Checklist of Gargaphia species from Colombia and its host plants (based on Drake and Ruhoff 1965; Posada et al. 1976; Madrigal 1978; Gallego and Vélez 1992; Guidoti et al. 2014; and data from CTNI, LEUA, and UNAB). Species Gargaphia acmonis Drake & Hambleton, 1945 Gargaphia concursa Drake, 1930 Gargaphia crotonae Drake & Hambleton, 1938 Gargaphia deceptiva (Drake & Bruner, 1924) Gargaphia lunulata Mayr, 1865 Gargaphia nigrinervis Stal, 1873 Gargaphia opima Drake, 1931 Gargaphia paula Drake, 1939 Gargaphia patricia Stal, 1862 Gargaphia trichoptera Stal, 1873 Gargaphia sanchezi Froeschner, 1972 Distribution Peru, Colombia, and Suriname Brazil and Colombia Brazil and Colombia British Guiana, Trinidad, Venezuela, and Colombia Argentina, Colombia, Brazil, Paraguay, and Uruguay Colombia, Venezuela, Peru, Panama, Guatemala, Aruba, and Curacao Peru, Colombia, and Bolivia Costa Rica, Ecuador, Panama, Brazil, and Peru; Colombia (current work) (Fig. 4). Mexico, Costa Rica, Guatema- la, Panama, Colombia, Venezu- ela, and Argentina Colombia, Peru, and Brazil Colombia Host plant Abelmoschus esculentus (L.) Moench (Malvaceae) Croton sp. (Euphorbiaceae), Annonaceae, and Phaseolus vulgaris L. (Fabaceae) Croton urucurana Baill. (Euphorbiaceae), Solanum sp. (Solanaceae), and unidenti- fied weed Ipomoea sp. (Convolvulaceae) Abelmoschus esculentus (L.) Moench (Malvaceae), Canavalia ensiformis (L.) DC. (Fabaceae), Cassia fistula L. (Fabaceae), Chorisia speciosa A. St.-Hil. (Malvaceae), Cnidoscolus sp. (Euphorbiaceae), Dolichos lablab L. (Fabaceae), Euphorbia hetero- phylla L. (Euphorbiaceae), Glycine max (L.) Merr. (Fabaceae), Gossypium arboreum L. (Malvaceae), Hibiscus esculentus L. (Malvaceae), Manihot utilissima Pohl (Euphorbia- ceae), Meibomia adscendens (Sw.) Kuntze (Fabaceae), Passiflora caerulea L. (Passiflo- raceae), Passiflora x violacea Loiseleur-Deslongchamps (Passifloraceae), Passiflora edulis Sims (Passifloraceae), Phaseolus lunatus L. (Fabaceae), Phaseolus vulgaris L. (Fabaceae), Psidium sp. (Myrtaceae), Pyrus communis L. (Rosaceae), Ricinus commu- nis L. (Euphorbiaceae), Rosa sp. (Rosaceae), Stigmaphyllon sp. (Malpighiaceae), Ure- na lobata L. (Malvaceae), and Zornia diphylla (L.) Pers. (Fabaceae) Jatropha urens L. (Euphorbiaceae), Solanum sp. (Solanaceae), and Phaseolus vulgar- is L. (Fabaceae) Canavalia ensiformis (L.) DC. (Fabaceae) Arachis pintoi Krapov. & W.C. Greg. (Fabaceae) and Calopogonium mucunoides Desv. (Fabaceae) (current work, new host plant record) (Fig. 5) Euphorbiaceae No record Phaseolus vulgaris L. (Fabaceae) and Desmodium affine Schltdl. (Fabaceae) Calopogonium mucunoides (common names: kudzu, calopo) (Fig. 5) is recorded here as a new host plant of G. paula. This plant species is widely distributed in the tropics and farmers employ it to control weeds in plan- tations. In Colombia and other tropical countries, C. mucu- Acknowledgements We thank the Universidad de la Amazonia for financial support of the research “Curation of Hemiptera (Het- eroptera) insects - Laboratory of Entomology [Curadu- ria de insectos Hemiptera (Heteroptera) - Laboratorio de Entomologia]”, assigned to JG in his teaching work. noides and A. pintoi (common name: mani forrajero) are associated with pastures of Brachiaria spp. as a strategy to recover degraded areas in extensive double- purpose cattle production systems (meat and milk). The phytophagy of G. paula on plants of C. mucunoides and A. pintoi causes a decrease in quantity and quality of available biomass for livestock feed. The Naturalist portal (https://www.inaturalist.org/ observations/16094005; accessed on 2023-9-19) shows an apparent previous record of G. paula for Colombia. However, this is of a specimen collected in Costa Rica (Ulloa, Heredia). We also thank the “Jornada Docente” project of FS at the UNAB museum in the Facultad de Ciencias Agrarias, Universidad Nacional de Colombia. We thank Juan Arcos for his support in taking photographs and preparing the figures for publication. Finally, we thank the reviewers and academic editor for contributions to this work. Author Contributions Conceptualization: JG, CF, JQ, EVVN, YACG, FS. Data curation: JG, YACG, EVVN, FS. Formal analysis: JQ, FS, EVVN, JG, CF. Methodology: EVVN, YACG, FS, Gamboa et al. | Gargaphia paula from Colombia JG. Writing - original draft: JG, FS. Writing - review and editing: FS, JG. Visualization: FS, JG, YACG. References Drake CJ (1939) Two new tingitids (Hemiptera) from Pan- ama. Psyche: a Journal of Entomology 46: 68-69. https:// doi.org/10.1155/1939/91214 Drake CJ, Ruhoff FA (1960) Lace-bug genera of the world (Hemiptera: Tingidae). 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Micropezidae (Diptera: Nerioidea) of the Entomolo- gical Museum UNAB. Agronomia Colombiana 33: 339- 347. https://doi.org/10.15446/agron.colomb.v33n3.52432 Morrone JJ (2014) Biogeographical regionalisation of the Neotropical region. Zootaxa 3782: 1-110. https://doi. org/10.11646/zootaxa.3782.1.1 Posada L, de Polania IZ, de Arévalo IS, Saldarriaga A, Garcia F, Cardenas R (1976) Lista de insectos daninos y otras plagas de Colombia. ICA, Bogota D.C., Colombia, 482 pp. Smithsonian Institution, National Museum of Natural History (2023) Gargaphia paula Drake, 1939. In: NUNH Extant Specimen Records (USNM, US). Version 1.69. https://www.gbif.org/occurrence/1321645521. Accessed on: 2023-6-30. National Museum of Natural History, Smith- sonian Institution, Washington DC, USA. https://doi.org/ 10.15468/hnhrg3